The Recombinant Fibrillarin Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110695) against Fibrillarin. This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of 3T3-L1 and IHC analysis of Mouse testis tissue, Rat liver tissue. The target is localized to the nucleus.
Product Name:
Recombinant Fibrillarin Monoclonal Antibody
SKU:
AGEL6004
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
FBL, FIB, FIB1, fibrillarin, FLRN, Nop1, RNU3IP1
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human Fibrillarin protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110695
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Fibrillarin
Conjugation:
Unconjugated
Observed MW:
34 kDa
Calculated MW:
34 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P22087
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded mouse testis tissue using Recombinant Fibrillarin Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded rat liver tissue using Recombinant Fibrillarin Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant Fibrillarin Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: 3T3-L1 cells. Observed-MW:34 kDa Calculated-MW:34 kDa