The Recombinant GATA1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C321549) against GATA1. GATA1 is the founding member of the GATA family and is required for erythroid and megakaryocytic cell development. Mutations in the corresponding GATA1 gene are linked to many human diseases, including acute megakaryoblastic leukemia in Down Syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB and IHC applications. It has been validated by WB analysis of HeLa (Negative control), K562, TF-1 and IHC analysis of Human colon, Human pancreas(Negative tissue). The target is localized to the nucleus.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
dilutionImmunohistochemistry of paraffin-embedded Human colon using GATA1 Monoclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Human pancreas(Negative tissue) using GATA1 Monoclonal Antibody at dilution of 1:2000.
Western Blot with GATA1 Monoclonal Antibody at of 1:1000. Lane 1: HeLa (Negative control), Lane 2: K562, Lane 3: TF-1 Observed-MW:53 kDa Calculated-MW:43 kDa