Cartilage Oligomeric Matrix Protein (COMP), also referred to as Thrombospondin-5, is a non-collagenous extracellular matrix (ECM) protein and belongs to the subgroup B of the thrombospondin protein family. This protein is expressed primarily in cartilage, ligament, and tendon, and binds to other ECM proteins such as collagen I, II and IX with high affinities depending on the divalent cations Zn2+ or Ni2+. COMP is a secreted glycoprotein that is important for growth plate organization and function. It is suggested to play a role in cell growth and development, and recent studies have revealed the possible mechanism that it protects cells against death by elevating members of the IAP (inhibitor of apoptosis protein) family of survival proteins. Mutations in COMP cause two skeletal dysplasias, pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (EDM1), and up-regulated expression of COMP are observed in rheumatoid arthritis and certain carcinomas.
Product Name:
Human COMP Recombinant Protein (RPES1000)
Product Code:
RPES1000
Size:
100 µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
EDM1,EPD1,MED,PSACH,THBS5
Accession:
NP_000086.2
Sequence:
Met 1-Ala 757
Fusion tag:
C-His
Activity:
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. When cells are added to coated plates (5 µg/ml, 100 ?l/well), approximately 40% will adhere specifically after 60 minutes at 37 °C.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human COMP (NP_000086.2) precusor (Met 1-Ala 757) was expressed with a C-terminal polyhistidine tag.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
82.4 kDa
AP Mol Mass:
120-130 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
