Dopamine beta-hydroxylase/DbH oxidoreductase protein is a copper type II, ascorbate-dependent monooxygenase enzyme. The encoded protein, which is present in the adrenal medulla's neuroscretory vesicles and chromaffin granules, converts dopamine to norepinephrine, a hormone and major neurotransmitter of the sympathetic nervous system. Low levels of dopamine beta-hydroxylate is caused by mutations in dopamine beta-hydroxylase/DbH in humans. A deficiency of dopamine beta-hydroxylate is characterised by autonomic and cardiovascular dysfunction, including hypotension and ptosis. Dopamine beta-hydroxylase/DbH polymorphisms might be linked to a variety of mental disorders. Dopamine beta-hydroxylase deficiency and orthostatic hypotension 1 are two diseases linked to DBH.Dopamine beta-Hydroxylase Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Dopamine beta-Hydroxylase Recombinant Protein (RPES5077)
Product Code:
RPES5077
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
DBM
Accession:
P09172
Sequence:
Ser26-Gly603
Fusion tag:
N-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human DBH (P09172) (Ser26-Gly603) was expressed with an N-terminal polyhistidine tag.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
67.3 kDa
AP Mol Mass:
68 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
