Growth Arrest and DNA Damage-Inducible Protein GADD45 ? (GADD45A) is a member of the GADD45 family. GADD45A can be induced by UV irradiation; X-rays; growth arrest and alkylating agents; of which can be mediated by some kinases other than PKC. GADD45A can interact with MAPK14; GADD45GIP1; PCNA. In T-cells; GADD45A functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. GADD45A may affect PCNA interaction with some cell division protein kinase complexes; stimulating DNA excision repair in vitro and inhibits entry of cells into S phase.
Product Name:
Human GADD45A/DDIT Recombinant Protein (RPES2363)
Product Code:
RPES2363
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Growth Arrest and DNA Damage-Inducible Protein GADD45 Alpha, DNA Damage-Inducible Transcript 1 Protein, DDIT-1, GADD45A, DDIT1, GADD45
Accession:
P24522
Sequence:
Met1-Arg165
Fusion tag:
N-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human GADD45A alpha is produced by our E.coli expression system and the target gene encoding Met1-Arg165 is expressed with a 6His tag at the N-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
20.5 kDa
AP Mol Mass:
18 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
