Recombinant Human GADD45A/DDIT Protein (His & GST Tag) (RPES3234)



ELISA Kit Technical Manual

Recombinant Human GADD45A/DDIT Protein (His & GST Tag)

Background:GADD45A is a member of the GADD45 Family, and has been found to associate with several cytoplasmic and nuclear factors and has been implicated in several cellular functions, including MAPK signaling, cell cycle regulation, DNA repair and genomic stability, apoptosis, and immune responses. The GADD45 Family of genes is rapidly induced by different stressors, including differentiation-inducing cytokines, and there is a large body of evidence that their cognate proteins are key players in cellular stress responses. GADD45A protein has been reported to interact with multiple important cellular proteins, including Cdc2 protein kinase, proliferating cell nuclear antigen (PCNA), p21Waf1/Cip1 protein, core histone protein and MTK/MEKK4, an up-stream activator of the JNK/SAPK pathway, indicating that GADD45A may play important roles in the control of cell cycle checkpoint, DNA repair process, and signaling transduction. GADD45A expression in response to genotoxic stress illustrates a more complex scenario, wherein transcriptional changes operate in concert with mRNA turnover and translational regulation. GADD45A was the first stress-inducible gene determined to be up-regulated by p53 and is also a target for the p53 homologues, p63 and p73. The decreased GADD45A expression is also considered a survival mechanism, as cancer cells without this control can evade the apoptotic pathway leading to increased tumourigenesis. As GADD45A is an essential component of many metabolic pathways that control proliferating cancer cells, it presents itself as an emerging drug target worthy of further investigation.
Name:Recombinant Human GADD45A/DDIT Protein (His & GST Tag)
Assay Genie SKU:RPES3234
Synonyms:Growth Arrest and DNA Damage-Inducible Protein GADD45 Alpha; DNA Damage-Inducible Transcript 1 Protein; DDIT; GADD45A; DDIT1; GADD45
Expression host:Baculovirus-Insect Cells
Sequence:Met 1-Arg 165
Mol Mass:46.2 kDa
AP Mol Mass:46 kDa
Tag:N-His & GST
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:GADD45A: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Interacts with MAPK14. Interacts with GADD45GIP1. Interacts with PCNA. By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediate by some kinase(s) other than PKC. Belongs to the GADD45 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; DNA repair, damage; Apoptosis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p31.2

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function:protein binding

Biological Process: activation of MAPKKK activity; apoptosis; cell cycle arrest; DNA damage response, signal transduction; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; positive regulation of apoptosis; positive regulation of JNK cascade; regulation of cyclin-dependent protein kinase activity

NCBI Summary:This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]
UniProt Code:P24522
NCBI GenInfo Identifier:120751
NCBI Gene ID:1647
NCBI Accession:P24522.1
UniProt Secondary Accession:P24522,Q5TCA7, Q5TCA8,
UniProt Related Accession:P24522
Molecular Weight:14,622 Da
NCBI Full Name:Growth arrest and DNA damage-inducible protein GADD45 alpha
NCBI Synonym Full Names:growth arrest and DNA damage inducible alpha
NCBI Official Symbol:GADD45A
NCBI Official Synonym Symbols:DDIT1; GADD45
NCBI Protein Information:growth arrest and DNA damage-inducible protein GADD45 alpha
UniProt Protein Name:Growth arrest and DNA damage-inducible protein GADD45 alpha
UniProt Synonym Protein Names:DNA damage-inducible transcript 1 protein; DDIT
Protein Family:Growth arrest and DNA damage-inducible protein
UniProt Gene Name:GADD45A
UniProt Entry Name:GA45A_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Baculovirus-Insect Cells
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