GADD45A is a member of the GADD45 Family; and has been found to associate with several cytoplasmic and nuclear factors and has been implicated in several cellular functions; including MAPK signaling; cell cycle regulation; DNA repair and genomic stability; apoptosis; and immune responses. The GADD45 Family of genes is rapidly induced by different stressors; including differentiation-inducing cytokines; and there is a large body of evidence that their cognate proteins are key players in cellular stress responses. GADD45A protein has been reported to interact with multiple important cellular proteins; including Cdc2 protein kinase; proliferating cell nuclear antigen (PCNA); p21Waf1/Cip1 protein; core histone protein and MTK/MEKK4; an up-stream activator of the JNK/SAPK pathway; indicating that GADD45A may play important roles in the control of cell cycle checkpoint; DNA repair process; and signaling transduction. GADD45A expression in response to genotoxic stress illustrates a more complex scenario; wherein transcriptional changes operate in concert with mRNA turnover and translational regulation. GADD45A was the first stress-inducible gene determined to be up-regulated by p53 and is also a target for the p53 homologues; p63 and p73. The decreased GADD45A expression is also considered a survival mechanism; as cancer cells without this control can evade the apoptotic pathway leading to increased tumourigenesis. As GADD45A is an essential component of many metabolic pathways that control proliferating cancer cells; it presents itself as an emerging drug target worthy of further investigation.
Product Name:
Human GADD45A/DDIT Recombinant Protein (RPES3234)
Product Code:
RPES3234
Size:
20µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
Growth Arrest and DNA Damage-Inducible Protein GADD45 Alpha, DNA Damage-Inducible Transcript 1 Protein, DDIT-1, GADD45A, DDIT1, GADD45
Accession:
P24522
Sequence:
Met 1-Arg 165
Fusion tag:
N-His & GST
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the full length of human GADD45A (P24522) (Met 1-Arg 165) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
