PGA4 (Pepsinogen 4; group I); or Pepsinogen A; is a member of the peptidase A1 family. Pepsin is expressed as a pro-form zymogen; pepsinogen; whose primary structure has an additional 44 amino acids. Pepsin is stored as pepsinogen so it will only be released when needed; and does not digest the body's own proteins in the stomach's lining. Five types of zymogens of pepsins; gastric digestive proteinases; are known: pepsinogens A; B; and F; progastricsin; and prochymosin. There are two major groups of pepsinogen; namely pepsinogen A (PGA) and pepsinogen C (PGC) (or progastricsin); and each frequently has isozymogens. The PGA3; PGA4 and PGA5 genes encode identical human pepsinogen A enzymes.
Product Name:
Human PGA4/Pepsinogen A Recombinant Protein (RPES1341)
Product Code:
RPES1341
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
FLJ58952,FLJ77962,PGA3,PGA4,PGA5
Accession:
P00790
Sequence:
Met 1-Ala 388
Fusion tag:
C-His
Activity:
Measured by its ability to cleave the fluorogenic peptide substrate; Mca-RPKPVE-Nval-WRK(Dnp)-NH2; AnaSpec; Catalog # 27114.The specific activity is > 10;000 pmoles/min/µg.(Activation description: The enzyme achieves its activity under acidic pH)
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human PGA4 (NP_001073276.1) (Met 1-Ala 388) was fused with a polyhistidine tag at the C-terminus.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
41.7 kDa
AP Mol Mass:
45 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
