HB-GAM belongs to the pleiotrophin family. HB-GAM is expressed in the central and peripheral nervous systems, as well as numerous non-neural tissues such as the lung, kidney, gut, and bone throughout embryonic and early postnatal development. It is expressed in an activity-dependent manner in the hippocampus of adult mice, where it can reduce long-term potentiation induction. In the adult brain, HB-GAM is low overall but can be induced by ischemic insults or targeted neuronal damage to the entorhinal cortex or substantia nigra pars compact (SNpc). It has a high affinity for heparin and is structurally related to retinoic acid-induced heparin-binding protein and midkine. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also has a mitogenic effect on fibroblasts, epithelial, and endothelial cells as a secreted growth factor. Pleiotrophin/PTN/HB-GAM Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Pleiotrophin/PTN/HB-GAM Recombinant Protein (RPES4776)
Product Code:
RPES4776
Size:
5µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
HARP,HBGF8,HBNF,NEGF1
Accession:
NP_002816.1
Sequence:
Met 1-Asp168
Fusion tag:
Activity:
Measured by its binding ability in a functional ELISA. Immobilized human PTN at 10 µg/ml (100 ?l/well) can bind rat SDC1-Fc, The EC50 of rat SDC1-Fc is 0.35-0.81 µg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mature form of human PTN (NP_002816.1) (Met1-Asp168) was expressed.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
15.3 kDa
AP Mol Mass:
19 kDa
Formulation:
Lyophilized from sterile 20mM Tris, 1M NaCl, pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
