PPP3R1 is an active regulatory subunit of calcineurin. Calcineurin is a calcium-dependent, calcium-modulated protein phosphatase. Calcium sensitivity is modified by PPP3R1. Spinocerebellar ataxia is a PPP3R1-related condition. The phospholipase C pathway, CDK-mediated phosphorylation, and Cdc6 removal are all examples of PPP3R1 related pathways. Human PPP3R1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human PPP3R1 Recombinant Protein (RPES3670)
Product Code:
RPES3670
Size:
100 µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
Calcineurin Subunit B Type 1, Protein Phosphatase 2B Regulatory Subunit 1, Protein Phosphatase 3 Regulatory Subunit B Alpha Osoform 1, PPP3R1, CNA2, CNB
Accession:
P63098
Sequence:
Gly2-Val170
Fusion tag:
N-His
Activity:
Using the Octet RED System; the affinity constant (Kd) of human PPP3R1-His bound to Human PPIA-His was 6 nM.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human PPP3R1 (P63098) (Gly2-Val170) was fused with a polyhistide tag at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
