The Recombinant IL-12 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C361187) against IL-12. Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for IF and FCM applications. It has been validated by IF analysis of PBMC and FCM analysis of PBMCs. The target is localized to the secreted.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human IL-12 expression on PBMCs cells. Cells were stained with purified anti-Human IL-12, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescent analysis of (4% Paraformaldehyde) fixed PBMC cells using anti-IL-12 Monoclonal Antibody at dilution of 1:50.
