CTSD localizes to the lysosome and consists of a light chain and a heavy chain. CTSD is expressed in epithelial cells as well as in macrophages.CTSD is a lysosomal aspartyl protease that depends critically on protonation of its active site Asp residue and gets activated at pH 5 in endosome of hepatocytes. It has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix and xonnective tissue. In addition, CTSD has been used as a breast cancer tumor marker.
Product Name:
Mouse Cathepsin D/CTSD Recombinant Protein (RPES0497)
Product Code:
RPES0497
Size:
50 µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Cathepsin D, CTSD,CPSD
Accession:
NP_034113.1
Sequence:
Ile21-Leu410
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse Cathepsin D is produced by our Mammalian expression system and the target gene encoding Ile21-Leu410 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
43.9 kDa
AP Mol Mass:
56kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM MES,150mM NaCl,pH5.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
