Lymphocyte-activation gene 3 (LAG3), also known as CD223, is a type I transmembrane protein with four extracellular Ig-like domains, designated D1 to D4 and belongs to the immunoglobulin superfamily. The gene for LAG3 lies adjacent to the gene for CD4 on human chromosome 12p13.32 and shares approximately 20% identical to the CD4 gene. LAG3 is expressed on activated T cells, natural killer cells, B cells and plasmacytoid dendritic cells. LAG3 binds with high affinity to MHC class II molecules, and it interferes competitively with the binding of CD4 to MHC class II and thereby blocks the transduction of stimulatory signals mediated by this interaction. LAG3 negatively regulates cellular proliferation, activation, and homeostasis of T cells, and plays an important role in Treg suppressive function. LAG3 is the target of various drug development programs to develop new treatments for cancer and autoimmune disorders. The soluble form, sLAG-3, is being developed as a cancer drug.
Product Name:
Mouse LAG3 Recombinant Protein (RPES2171)
Product Code:
RPES2171
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Lymphocyte activation gene 3 protein,CD223,Lag3
Accession:
Q61790
Sequence:
Ser23-Leu442
Fusion tag:
C-6His
Activity:
Immobilized Mouse LAG-3-His at 10µg/ml(100 ?l/well) can bind Biotinylated Human FGL1-Avi. The ED50 of Mouse LAG-3-His is 9.4 ug/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse Lymphocyte Activation Gene 3 Protein is produced by our Mammalian expression system and the target gene encoding Ser23-Leu442 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
46.2 kDa
AP Mol Mass:
55-80 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
