The Recombinant Phospho-Chk1 (Ser345) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C170947) against Chk1 (Ser345). The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IF and ELISA applications. It has been validated by WB analysis of NIH-3T3. The target is localized to the nucleus, chromosome, cytoplasm, cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Nuclear export is mediated at least in part by XPO1/CRM1 (PubMed:12676962). Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B (PubMed:15311285). Proteolytic cleavage at the C-terminus by SPRTN promotes removal from chromatin (PubMed:31316063).
A synthetic peptide corresponding to residues around (Ser345) of Human Phospho-Chk1
Applications:
WBIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C170947
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Chk1 (Ser345)
Conjugation:
Unconjugated
Observed MW:
56 kDa
Calculated MW:
56 kDa
Recommended Dilution:
WB 1:500-2000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
O14757
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant Phospho-Chk1 (Ser345) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: NIH-3T3 cell lysate. Observed-MW:56 kDa Calculated-MW:56 kDa
