The Recombinant Phospho-Rpb1 (Ser2) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C111022) against Rpb1 (Ser2). This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by IHC analysis of Rat spleen. The target is localized to the nucleus, cytoplasm, chromosome, hypophosphorylated form is mainly found in the cytoplasm, while the hyperphosphorylated and active form is nuclear (PubMed:26566685). Co-localizes with kinase SRPK2 and helicase DDX23 at chromatin loci where unscheduled R-loops form (PubMed:28076779).
![Anti-Phospho-POLR2A (Ser2) [R06-5Y1] Monoclonal Antibody (AGMB05321)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/276606/679012/anti-phospho-polr2a-ser2-r06-5y1-monoclonal-antibody-agmb05321__58805.1773037582.jpg?c=2 "Anti-Phospho-POLR2A (Ser2) [R06-5Y1] Monoclonal Antibody (AGMB05321)")
