The Recombinant Phospho-PERK (Thr980) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C471011) against PERK (Thr980). The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Mouse and Rat samples.
This antibody is suitable for WB, IF and ELISA applications. It has been validated by WB analysis of NIH/3T3. The target is localized to the Endoplasmic reticulum membrane; Single-pass type I membrane protein.
A synthetic peptide corresponding to residues around (Thr980) of Mouse Phospho-PERK
Applications:
WBIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C471011
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
PERK (Thr980)
Conjugation:
Unconjugated
Observed MW:
125 kDa
Calculated MW:
125 kDa
Recommended Dilution:
WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
Q9Z2B5
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant Phospho-PERK (Thr980) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: NIH/3T3 lysate. Observed-MW:125 kDa Calculated-MW:125 kDa
