The Recombinant Phospho-STAT5 (Tyr694) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110315) against STAT5 (Tyr694). The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Hela. The target is localized to the cytoplasm, nucleus.
Mammary gland factor, MGF, Signal transducer and activator of transcription 5A, STA5A, STAT 5, STAT 5A, STAT5, STAT5A, STAT5A/B
Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to residues around (Tyr694) of Human Phospho-STAT5
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110315
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
STAT5 (Tyr694)
Conjugation:
Unconjugated
Observed MW:
105 kDa
Calculated MW:
91 kDa
Recommended Dilution:
IHC 1:100-500; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P42229, P51692
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant Phospho-STAT5 (Tyr694) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Hela whole cell lysate. Observed-MW:105 kDa Calculated-MW:91 kDa
![Anti-Phospho-STAT5a (Tyr694) [R02-9B2] Monoclonal Antibody (AGMB05113)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/276398/679701/anti-phospho-stat5a-tyr694-r02-9b2-monoclonal-antibody-agmb05113__37366.1773039747.jpg?c=2 "Anti-Phospho-STAT5a (Tyr694) [R02-9B2] Monoclonal Antibody (AGMB05113)")
