The Recombinant Phospho-Synapsin-1 (Ser9) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C431723) against Synapsin-1 (Ser9). Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It isupregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complexwhich blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Mouse and Rat samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of Mouse brain, Rat brain. The target is localized to the mitochondrion matrix.
Brain protein, Brain protein 4.1, SYN, SYN 1, SYN 1a, SYN 1b, SYN I, SYN1, SYN1a, SYN1b, Synapsin 1, Synapsin I, Synapsin-1, Synapsin1, SynapsinI, SYNI
Reactivity:
Mouse, Rat
Immunogen:
Peptide. This information is proprietary to PTMab
Applications:
WB
Clonality:
Recombinant Monoclonal
Clone No:
C431723
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Synapsin-1 (Ser9)
Conjugation:
Unconjugated
Observed MW:
85 kDa
Calculated MW:
74 kDa
Recommended Dilution:
WB 1:1000
UniProt:
P17600
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Phospho-Synapsin-1 (Ser9) Monoclonal Antibody at dilution of 1:1000. Lane 1: Mouse brain, then the membrane treated with lambda protein phosphatase (400 U/ml) at 30°C for 1 hour, Lane 2: Mouse brain, Lane 3: Rat brain, then the membrane treated with lambda protein phosphatase (400 U/ml) at 30°C for 1 hour, Lane 4: Rat brain Observed-MW:85 kDa Calculated-MW:74 kDa