The Recombinant Shh Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110872) against Shh. This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by IHC analysis of Human hepatocellular carcinoma. The target is localized to the endoplasmic reticulum membrane, Golgi apparatus membrane, Co-localizes with HHAT in the ER and Golgi membrane, cell membrane, lipid-anchor, The dual-lipidated sonic hedgehog protein N-product (ShhNp) is firmly tethered to the cell membrane where it forms multimers (PubMed:24522195). Further solubilization and release from the cell surface seem to be achieved through different mechanisms, including the interaction with DISP1 and SCUBE2, movement by lipoprotein particles, transport by cellular extensions called cytonemes or by the proteolytic removal of both terminal lipidated peptides (PubMed:26875496, PubMed:24522195).
Product Name:
Recombinant Shh Monoclonal Antibody
SKU:
AGEL6178
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
HHG 1, HHG-1, HHG1, HLP, HLP 3, HLP3, Holoprosencephaly 3, HPE, HPE 3, HPE3, MCOPCB, MCOPCB5, SHH, SMMC I, SMMCI, Sonic hedgehog, Sonic Hedgehog (Drosophila) homolog, Sonic hedgehog homolog, sonic hedgehog homolog (Drosophila), Sonic Hedgehog Protein, Sonic hedgehog protein C-product, TPT, TPTPS
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human Shh protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110872
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Shh
Conjugation:
Unconjugated
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
Q15465
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
