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Recombinant U2AF2 Monoclonal Antibody (AGEL6984)

Datasheet MSDS
SKU AGEL6984
Product Type Antibody
Reactivity Human, Rat, Mouse
Applications WB, IHC, ChIP
Host Species Rabbit
Clonality Monoclonal
Clone C121679
Conjugate Unconjugated
$606 - $843

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Description

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Recombinant U2AF2 Monoclonal Antibody

The Recombinant U2AF2 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C121679) against U2AF2. TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaborationwith TFIIB. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.

This antibody is suitable for WB, IHC and ChIP applications. It has been validated by WB analysis of HEK-293, HeLa, Jurkat, NIH-3T3, IHC analysis of Human colon cancer, Mouse liver, Rat cerebrum and ChIP analysis of HepG2. The target is localized to the nucleus.

Product Name:Recombinant U2AF2 Monoclonal Antibody
SKU:AGEL6984
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:U2AF2, U2AF65
Reactivity:Human, Mouse, Rat
Immunogen:Peptide. This information is proprietary to PTMab.
Applications:WBIHCChIP
Clonality:Recombinant Monoclonal
Clone No:C121679
Host:Rabbit
Isotype:Rabbit IgG
Target:U2AF2
Conjugation:Unconjugated
Observed MW:65 kDa
Calculated MW:54 kDa
Recommended Dilution:WB 1:5000-10000; IHC 1:200; ChIP 6 µg/ 2×10 6 cells
UniProt:P26368
Purification:Protein A
Concentration:1 mg/mL
Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Product ImageChromatin immunoprecipitation analysis of HepG2 immunoprecipitated DNA by real-time PCR using primers specific for the human ZNF446-E and PPCS-I. The data are presented as enrichment of each sample relative to the total amount of input chromatin at each amplicon.
Product ImageImmunohistochemistry of paraffin-embedded Human colon cancer using U2AF2 Monoclonal Antibody at dilution of 1:200.
Product ImageImmunohistochemistry of paraffin-embedded Mouse liver using U2AF2 Monoclonal Antibody at dilution of 1:200.
Product ImageImmunohistochemistry of paraffin-embedded Rat cerebrum using U2AF2 Monoclonal Antibody at dilution of 1:200.
Product ImageWestern Blot with U2AF2 Monoclonal Antibody at dilution of 1:10000. Lane 1: HEK-293, Lane 2: HeLa, Lane 3: Jurkat, Lane 4: NIH-3T3 Observed-MW:65 kDa Calculated-MW:54 kDa
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