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Key Features

Recombinant Vinculin Monoclonal Antibody (AGEL8585)

Datasheet MSDS
SKU AGEL8585
Product Type Antibody
Reactivity Human, Mouse, Rat
Applications WB, IF
Host Species Rabbit
Clonality Monoclonal
Clone C173280
Conjugate Unconjugated
$461 - $632

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Description

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Recombinant Vinculin Monoclonal Antibody

The Recombinant Vinculin Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C173280) against Vinculin. Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Produced by recombinant technology in Rabbit hosts and purified by affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.

This antibody is suitable for WB and IF applications. It has been validated by WB analysis of K562, Rat Rat Brain, C6, 3T3, Hela and IF analysis of Hela. The target is localized to the Cytoplasm>cytoskeleton. Cell junction>adherens junction. Cell membrane. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.

Product Name:Recombinant Vinculin Monoclonal Antibody
SKU:AGEL8585
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:CMD1W, CMH15, Epididymis luminal protein 114, HEL114, Metavinculin, MV, MVCL, OTTHUMP00000019861, OTTHUMP00000019862, VCL, VINC, Vinculin
Reactivity:Human, Mouse, Rat
Immunogen:A synthetic peptide of human Vinculin
Applications:WBIF
Clonality:Recombinant Monoclonal
Clone No:C173280
Host:Rabbit
Isotype:Rabbit IgG
Target:Vinculin
Conjugation:Unconjugated
Observed MW:124 kDa
Calculated MW:124 kDa
Recommended Dilution:WB 1:1000-2000; IF 1:50-100
UniProt:P18206
Purification:Affinity purification
Concentration:0.3 mg/mL
Buffer:50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
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