The Recombinant Vitronectin/S-Protein Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C311309) against Vitronectin/S-Protein. Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB, IHC, IF and FCM applications. It has been validated by WB analysis of Human serum, IHC analysis of Human liver, IF analysis of A549, HepG2 and FCM analysis of HepG2. The target is localized to the secreted.
WB 1:500-2000; IHC 1:50-100; IF 1:50; FCM 1:50-100
UniProt:
P04004
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human Vitronectin/S-Protein expression on HepG2 cells. Cells were stained with purified anti-Human Vitronectin/S-Protein, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescent analysis of (4% Paraformaldehyde) fixed A549 cells using anti-Vitronectin/S-Protein Monoclonal Antibody at dilution of 1:50.
Immunofluorescent analysis of (4% Paraformaldehyde) fixed HepG2 cells using anti-Vitronectin/S-Protein Monoclonal Antibody at dilution of 1:50.
Immunohistochemistry of paraffin-embedded Human liver using Vitronectin/S-Protein Monoclonal Antibody at dilution of 1:100.
Western Blot with Vitronectin/S-Protein Monoclonal Antibody at dilution of 1:2000. Lane 1: Human serum Observed-MW:75 kDa Calculated-MW:54 kDa