Recombinant Human Vitronectin/VTN Protein (His Tag)(Active) (RPES2626)



ELISA Kit Technical Manual

Recombinant Human Vitronectin/VTN Protein (His Tag)(Active)

Background:Human Vitronectin/VTN is a cell adhesion and spreading factor. It can be found in the blood and the extracellular matrix (ECM). Vitronectin interacts with glycosaminoglycans and proteoglycans. The multimeric Vitronectin can efficiently bind to and incorporate into the ECM; Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Vitronectin can be recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecular. It can as a inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Vitronectin contains an endogenous cleavage site, plus cleavage sites for elastase, thrombin, and plasmin.
Name:Recombinant Human Vitronectin/VTN Protein (His Tag)(Active)
Assay Genie SKU:RPES2626
Synonyms:Vitronectin; VN; S-Protein; Serum-Spreading Factor; V75; VTN
Expression host:Human Cells
Mol Mass:53.4 kDa
AP Mol Mass:59 kDa
Bio Activity:Measured by the ability of the immobilized protein to support the adhesion of Jurkat cells.
Purity:> 90 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:VTN: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 17q11

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function:heparin binding; integrin binding; protein binding; extracellular matrix binding; scavenger receptor activity; polysaccharide binding

Biological Process: receptor-mediated endocytosis; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell-matrix adhesion; negative regulation of blood coagulation; positive regulation of smooth muscle cell migration; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of receptor-mediated endocytosis; positive regulation of peptidyl-tyrosine phosphorylation; regulation of complement activation; cell adhesion mediated by integrin; innate immune response; immune response; cell adhesion

NCBI Summary:The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]
UniProt Code:P04004
NCBI GenInfo Identifier:139653
NCBI Gene ID:7448
NCBI Accession:P04004.1
UniProt Related Accession:P04004
Molecular Weight:
NCBI Full Name:Vitronectin
NCBI Synonym Full Names:vitronectin
NCBI Official Symbol:VTN  
NCBI Official Synonym Symbols:VN; V75; VNT  
NCBI Protein Information:vitronectin
UniProt Protein Name:Vitronectin
UniProt Synonym Protein Names:S-protein; Serum-spreading factor; V75Cleaved into the following 3 chains:Vitronectin V65 subunit; Vitronectin V10 subunit; Somatomedin-B
Protein Family:Vitronectin
UniProt Gene Name:VTN  
UniProt Entry Name:VTNC_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
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