The Recombinant WT1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C121643) against WT1. Wilms' Tumor protein (WT1) is a transcription factor named from Wilms' Tumor 1, an embryonal malignancy of the kidneys that is caused by mutations in the WT1 gene. It is highly important in development, particularly of the genitourinary system, and mutations and dysregulation of expression of WT1 result in a variety of syndromes affecting the genitourinary system and other tissues.WT1 has a myriad of biological functions and a host of interacting partners and target genes. It can behave as a transcriptional activator, or a repressor, and can act as an oncogene or a tumor suppressor. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB and IHC applications. It has been validated by WB analysis of LNCaP (negative?control),?K562, Mouse testis,?Rat testis and IHC analysis of Human kidney, Human testis, Human Wilms tumor, Mouse testis. The target is localized to the cytoplasm, nucleus.
Peptide. This information is proprietary to PTMab.
Applications:
WBIHC
Clonality:
Recombinant Monoclonal
Clone No:
C121643
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
WT1
Conjugation:
Unconjugated
Observed MW:
52 kDa
Calculated MW:
49 kDa
Recommended Dilution:
WB 1:2000-10000; IHC 1:100
UniProt:
P19544
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded Human kidney using WT1 Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Human testis using WT1 Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Human Wilms tumor using WT1 Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Mouse testis using WT1 Monoclonal Antibody at dilution of 1:100.
Western Blot with WT1 Monoclonal Antibody at dilution of 1:10000. Lane 1: LNCaP (negative control), Lane 2: K562 Observed-MW:52 kDa Calculated-MW:49 kDa
Western Blot with WT1 Monoclonal Antibody at dilution of 1:1000. Lane 1: Mouse testis, Lane 2: Rat testis Observed-MW:52 kDa Calculated-MW:49 kDa
