The Recombinant YB-1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110808) against YB-1. This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of MCF7 and IHC analysis of Human prostate carcinoma. The target is localized to the cytoplasm, nucleus, cytoplasmic granule, secreted, secreted, extracellular exosome, Predominantly cytoplasmic in proliferating cells (PubMed:12604611). Cytotoxic stress and DNA damage enhance translocation to the nucleus (PubMed:14718551). Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:25229427). Shuttles between nucleus and cytoplasm (PubMed:25229427). Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress (PubMed:18335541). Secreted by mesangial and monocytic cells after inflammatory challenges (PubMed:19483673).
Product Name:
Recombinant YB-1 Monoclonal Antibody
SKU:
AGEL6115
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
BP 8, BP-8, CBF-A, CCAAT binding transcription factor I subunit A, CCAAT-binding transcription factor I subunit A, CSDA, CSDA2, CSDB, DBPB, DNA binding protein B, DNA-binding protein B, EFI-A, Enhancer factor I subunit A, MDR NF1, MDR-NF, MDR-NF1, MGC104858, MGC110976, MGC117250, NSEP 1, NSEP-1, NSEP1, Nuclease sensitive element binding protein 1, Nuclease-sensitive element-binding protein 1, p50, Q15905, Y-box binding protein 1, Y-box transcription factor, Y-box-binding protein 1, YB 1, YB-1, YB1, YBOX1, YBX, YBX 1, YBX1
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human YB-1 protein
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110808
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
YB-1
Conjugation:
Unconjugated
Observed MW:
49 kDa
Calculated MW:
36 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P67809
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
