Anti-RCVRN Antibody (CAB6404)

Antibody Name:	Anti-RCVRN Antibody
Antibody SKU:	CAB6404
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RCVRN (NP_002894.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse eye, Rat eye

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RCVRN (NP_002894.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGNS KSGA LSKE ILEE LQLN TKFS EEEL CSWY QSFL KDCP TGRI TQQQ FQSI YAKF FPDT DPKA YAQH VFRS FDSN LDGT LDFK EYVI ALHM TTAG KTNQ KLEW AFSL YDVD GNGT ISKN EVLE IVMA IFKM ITPE DVKL LPDD ENTP EKRA EKIW KYFG KNDD DKLT EKEF IEGT LANK EILR LIQF EPQK VKEK MKNA
Gene ID:	5957
Uniprot:	P35243
Cellular Location:
Calculated MW:	23kDa
Observed MW:	23kDa

Synonyms:	RCVRN, RCV1, recoverin
Background:	This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy.

UniProt Protein Function:	RCV1: Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse. Belongs to the recoverin family.
UniProt Protein Details:	Protein type:Calcium-binding Chromosomal Location of Human Ortholog: 17p13.1 Molecular Function:calcium ion binding; calcium sensitive guanylate cyclase activator activity Biological Process: phototransduction, visible light; positive regulation of guanylate cyclase activity; regulation of calcium ion transport; regulation of rhodopsin mediated signaling; rhodopsin mediated signaling; signal transduction; visual perception
NCBI Summary:	This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy. [provided by RefSeq, Jul 2008]
UniProt Code:	P35243
NCBI GenInfo Identifier:	464600
NCBI Gene ID:	5957
NCBI Accession:	P35243.2
UniProt Secondary Accession:	P35243,Q53XL0,
UniProt Related Accession:	P35243
Molecular Weight:	23,130 Da
NCBI Full Name:	Recoverin
NCBI Synonym Full Names:	recoverin
NCBI Official Symbol:	RCVRN
NCBI Official Synonym Symbols:	RCV1
NCBI Protein Information:	recoverin
UniProt Protein Name:	Recoverin
UniProt Synonym Protein Names:	Cancer-associated retinopathy protein; Protein CAR
Protein Family:	Recoverin
UniProt Gene Name:	RCVRN
UniProt Entry Name:	RECO_HUMAN

	Western blot analysis of extracts of various cell lines, using RCVRN antibody (CAB6404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded rat retina using RCVRN antibody (CAB6404) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse retina using RCVRN antibody (CAB6404) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of Y79 cells using RCVRN antibody (CAB6404) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
RCVRN Antibody (PACO03061)	Recoverin Colorimetric Cell-Based ELISA
RCVRN Antibody (PACO11782)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)