ROCK2 Rabbit Monoclonal Antibody (CAB2395)

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CAB2395
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Description

Technical Manual

ROCK2 Rabbit Monoclonal Antibody

Product Name:ROCK2 Rabbit Monoclonal Antibody
Product SKU:CAB2395
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

Immunogen:A synthesized peptide derived from human ROCK2
Sequence:

Email for sequence

Gene ID:9475
Swiss Prot:O75116
Synonyms:ROCK-II
Calculated MW:161kDa
Observed MW:160KDa
Reactivity:Human, Mouse, Rat
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry 
Positive Samples:HeLa, U-87MG, Mouse lung, Mouse brain, Rat brain
Cellular Location:

Product Images

ROCK2 Rabbit Monoclonal Antibody (CAB2395)

Western blot - ROCK2 Rabbit mAb (CAB2395)

Uniprot Information

UniProt Protein Function:ROCK2: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1. Activated by RHOA binding. Inhibited by Y-27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; AGC group; DMPK family; ROCK subfamily

Chromosomal Location of Human Ortholog: 2p24

Cellular Component: centrosome; plasma membrane; spindle pole centrosome; cytosol; nucleus

Molecular Function:protein serine/threonine kinase activity; protein binding; Rho GTPase binding; metal ion binding; structural molecule activity; ATP binding

Biological Process: regulation of cell adhesion; axon guidance; dendrite morphogenesis; rhythmic process; cytokinesis; regulation of circadian rhythm; protein amino acid phosphorylation; Rho protein signal transduction; negative regulation of angiogenesis; smooth muscle contraction; induction of apoptosis via death domain receptors; regulation of stress fiber formation; regulation of actin cytoskeleton organization and biogenesis; neural tube closure; regulation of focal adhesion formation; ephrin receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis; centrosome duplication; regulation of keratinocyte differentiation

NCBI Summary:The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]
UniProt Code:O75116
NCBI GenInfo Identifier:269849761
NCBI Gene ID:9475
NCBI Accession:O75116.4
UniProt Related Accession:O75116
Molecular Weight:
NCBI Full Name:Rho-associated protein kinase 2
NCBI Synonym Full Names:Rho-associated, coiled-coil containing protein kinase 2
NCBI Official Symbol:ROCK2 
NCBI Official Synonym Symbols:ROCK-II 
NCBI Protein Information:rho-associated protein kinase 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II
UniProt Protein Name:Rho-associated protein kinase 2
UniProt Synonym Protein Names:Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2
Protein Family:Rho-associated protein kinase
UniProt Gene Name:ROCK2 
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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