The ROCK2 Monoclonal Antibody (CAB2395) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blotting and immunofluorescence applications.ROCK2, a member of the Rho-associated coiled-coil containing protein kinase family, plays a key role in the regulation of actin cytoskeleton organization and cell motility. Dysregulation of ROCK2 signaling has been implicated in various diseases, including cancer and cardiovascular disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ROCK2 Monoclonal Antibody
SKU:
CAB2395
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0744
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
Purification Method
Affinity purification
Gene ID
9475
RRID
AB_2863000
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ROCK2 Rabbit mAb (CAB2395) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using ROCK2 Rabbit mAb (CAB2395) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
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