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Anti-RPA1 Antibody (CAB0990)

SKU:
CAB0990
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-RPA1 Antibody CAB0990
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-RPA1 Antibody CAB0990
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-RPA1 Antibody
Antibody SKU:CAB0990
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 367-616 of human RPA1 (NP_002936.1).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:U-87MG, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 367-616 of human RPA1 (NP_002936.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KFDG SRQP VLAI KGAR VSDF GGRS LSVL SSST IIAN PDIP EAYK LRGW FDAE GQAL DGVS ISDL KSGG VGGS NTNW KTLY EVKS ENLG QGDK PDYF SSVA TVVY LRKE NCMY QACP TQDC NKKV IDQQ NGLY RCEK CDTE FPNF KYRM ILSV NIAD FQEN QWVT CFQE SAEA ILGQ NAAY LGEL KDKN EQAF EEVF QNAN FRSF IFRV RVKV ETYN DESR IKAT VMDV KPVD YREY GRRL VMSI RRSA LM
Gene ID:6117
Uniprot:P27694
Cellular Location:Nucleus, PML body
Calculated MW:68kDa
Observed MW:68kDa
Synonyms:RPA1, HSSB, MST075, REPA1, RF-A, RP-A, RPA70
Background:
UniProt Protein Function:RPA1: Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: male germ cell nucleus; nucleoplasm; lateral element; PML body; DNA replication factor A complex; nucleus

Molecular Function:protein binding; metal ion binding; damaged DNA binding; chromatin binding; single-stranded DNA binding

Biological Process: meiosis; mismatch repair; in utero embryonic development; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; DNA recombination; telomere maintenance via semi-conservative replication; DNA recombinase assembly; homeostasis of number of cells within a tissue; base-excision repair; nucleotide-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; positive regulation of cell proliferation; telomere maintenance via recombination; hemopoiesis; nucleotide-excision repair, DNA damage removal; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA replication; DNA-dependent DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle

UniProt Code:P27694
NCBI GenInfo Identifier:1350579
NCBI Gene ID:6117
NCBI Accession:P27694.2
UniProt Secondary Accession:P27694,Q59ES9, A8K0Y9,
UniProt Related Accession:P27694
Molecular Weight:616
NCBI Full Name:Replication protein A 70 kDa DNA-binding subunit
NCBI Synonym Full Names:replication protein A1, 70kDa
NCBI Official Symbol:RPA1  
NCBI Official Synonym Symbols:HSSB; RF-A; RP-A; REPA1; RPA70; MST075  
NCBI Protein Information:replication protein A 70 kDa DNA-binding subunit; MSTP075; RP-A p70; RF-A protein 1; replication factor A protein 1; single-stranded DNA-binding protein
UniProt Protein Name:Replication protein A 70 kDa DNA-binding subunit
UniProt Synonym Protein Names:Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding proteinReplication protein A 70 kDa DNA-binding subunit, N-terminally processed
Protein Family:Replication factor
UniProt Gene Name:RPA1  
UniProt Entry Name:RFA1_HUMAN
Anti-RPA1 Antibody (CAB0990)Western blot analysis of extracts of various cell lines, using RPA1 antibody (CAB0990) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-RPA1 Antibody (CAB0990)Immunofluorescence analysis of MCF-7 cells using RPA1 antibody (CAB0990). Blue: DAPI for nuclear staining.

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