The RPF2 Antibody (CAB17224) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has been shown to have high reactivity with human samples. Validated for use in Western blot applications, the RPF2 Polyclonal Antibody binds specifically to the RPF2 protein, allowing for accurate detection and analysis in various cell types.Research into the function of RPF2 is important for understanding its role in protein synthesis and ribosome assembly, which are essential processes for cell growth and proliferation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RPF2 Antibody
SKU:
CAB17224
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BXDC1, bA397G5.4, RPF2
Positive Sample:
HeLa
Cellular Localization:
Nucleolus, Nucleoplasm.
Calculated MW:
36kDa
Observed MW:
36kDa
Enables 5S rRNA binding activity. Involved in protein localization to nucleolus; regulation of signal transduction by p53 class mediator; and ribosomal large subunit biogenesis. Located in chromosome; nucleolus; and nucleoplasm.
Purification Method
Affinity purification
Gene ID
84154
RRID
AB_2772069
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using RPF2 Rabbit pAb (CAB17224) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
