Anti-RPL23 Antibody (CAB4292)

Antibody Name:	Anti-RPL23 Antibody
Antibody SKU:	CAB4292
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human RPL23 (NP_000969.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:4000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, 293T, HeLa, HepG2, BT-474, HT-1080, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human RPL23 (NP_000969.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSKR GRGG SSGA KFRI SLGL PVGA VINC ADNT GAKN LYII SVKG IKGR LNRL PAAG VGDM VMAT VKKG KPEL RKKV HPAV VIRQ RKSY RRKD GVFL YFED NAGV IVNN KGEM KGSA ITGP VAKE CADL WPRI ASNA GSIA
Gene ID:	9349
Uniprot:	P62829
Cellular Location:
Calculated MW:	14kDa
Observed MW:	15kDa

Synonyms:

RPL23, L23, rpL17

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL17 because the encoded protein shares amino acid identity with ribosomal protein L17 from Saccharomyces cerevisiae; however, its official symbol is RPL23. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL23: a ribosomal protein of the L14P family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit.
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 17q Cellular Component: cytoplasm; cytosol; focal adhesion; membrane Molecular Function:protein binding; structural constituent of ribosome Biological Process: mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL17 because the encoded protein shares amino acid identity with ribosomal protein L17 from Saccharomyces cerevisiae; however, its official symbol is RPL23. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P62829
NCBI GenInfo Identifier:	51338639
NCBI Gene ID:	9349
NCBI Accession:	P62829.1
UniProt Secondary Accession:	P62829,P23131, P24048, Q29246, Q3SWV7, Q6P5S1,
UniProt Related Accession:	P62829
Molecular Weight:	14,865 Da
NCBI Full Name:	60S ribosomal protein L23
NCBI Synonym Full Names:	ribosomal protein L23
NCBI Official Symbol:	RPL23
NCBI Official Synonym Symbols:	L23; rpL17
NCBI Protein Information:	60S ribosomal protein L23
UniProt Protein Name:	60S ribosomal protein L23
UniProt Synonym Protein Names:	60S ribosomal protein L17
Protein Family:	50S ribosomal protein
UniProt Gene Name:	RPL23
UniProt Entry Name:	RL23_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL23 antibody (CAB4292) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat brain using RPL23 Rabbit pAb (CAB4292) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using RPL23 Rabbit pAb (CAB4292) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using RPL23 antibody (CAB4292) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using RPL23 antibody (CAB4292) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

RPL23 Antibody (PACO05748)

RPL23 Antibody (PACO11916)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)