Anti-RPL23A Antibody (CAB4086)

Antibody Name:	Anti-RPL23A Antibody
Antibody SKU:	CAB4086
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human RPL23A (NP_000975.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HT-1080, COS-7, HeLa, Jurkat, MCF7, Mouse lung, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human RPL23A (NP_000975.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPK AKKE APAP PKAE AKAK ALKA KKAV LKGV HSHK KKKI RTSP TFRR PKTL RLRR QPKY PRKS APRR NKLD HYAI IKFP LTTE SAMK KIED NNTL VFIV DVKA NKHQ IKQA VKKL YDID VAKV NTLI RPDG EKKA YVRL APDY DALD VANK IGII
Gene ID:	6147
Uniprot:	P62750
Cellular Location:
Calculated MW:	17kDa
Observed MW:	20kDa

Synonyms:

RPL23A, L23A, MDA20

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L23P family of ribosomal proteins. It is located in the cytoplasm. The protein may be one of the target molecules involved in mediating growth inhibition by interferon. In yeast, the corresponding protein binds to a specific site on the 26S rRNA. This gene is co-transcribed with the U42A, U42B, U101A, and U101B small nucleolar RNA genes, which are located in its third, first, second, and fourth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL23A: This protein binds to a specific region on the 26S rRNA. Belongs to the ribosomal protein L23P family.
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 17q11.2 Cellular Component: cytoplasm; nucleolus; TORC2 complex; nucleus; cytosol Molecular Function:rRNA binding; protein binding; structural constituent of ribosome; nucleotide binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; cell proliferation; cellular protein metabolic process; translational elongation; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; viral infectious cycle; translational termination
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L23P family of ribosomal proteins. It is located in the cytoplasm. The protein may be one of the target molecules involved in mediating growth inhibition by interferon. In yeast, the corresponding protein binds to a specific site on the 26S rRNA. This gene is co-transcribed with the U42A, U42B, U101A, and U101B small nucleolar RNA genes, which are located in its third, first, second, and fourth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P62750
NCBI GenInfo Identifier:	51338637
NCBI Gene ID:	6147
NCBI Accession:	P62750.1
UniProt Secondary Accession:	P62750,P29316, P39024, Q92774, B2R5B2,
UniProt Related Accession:	P62750
Molecular Weight:	156
NCBI Full Name:	60S ribosomal protein L23a
NCBI Synonym Full Names:	ribosomal protein L23a
NCBI Official Symbol:	RPL23A
NCBI Official Synonym Symbols:	L23A; MDA20
NCBI Protein Information:	60S ribosomal protein L23a; melanoma differentiation-associated gene 20
UniProt Protein Name:	60S ribosomal protein L23a
Protein Family:	60S ribosomal protein
UniProt Gene Name:	RPL23A
UniProt Entry Name:	RL23A_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL23A antibody (CAB4086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat brain using RPL23A Rabbit pAb (CAB4086) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using RPL23A Rabbit pAb (CAB4086) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using RPL23A Rabbit pAb (CAB4086) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using RPL23A Rabbit pAb (CAB4086) at dilution of 1:100 (40x lens).

Antibodies

RPL23A Antibody (PACO11917)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)