Anti-RPL28 Antibody (CAB15095)

Antibody Name:	Anti-RPL28 Antibody
Antibody SKU:	CAB15095
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPL28 (NP_000982.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, 293T, DU145, mouse kidney, mouse liver, rat spleen

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPL28 (NP_000982.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSAH LQWM VVRN CSSF LIKR NKQT YSTE PNNL KARN SFRY NGLI HRKT VGVE PAAD GKGV VVVI KRRS GQRK PATS YVRT TINK NARA TLSS IRHM IRKN
Gene ID:	6158
Uniprot:	P46779
Cellular Location:
Calculated MW:	7kDa/10kDa/15kDa/18kDa/19kDa
Observed MW:	16kDa

Synonyms:

RPL28, L28

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

UniProt Protein Function:	Component of the large ribosomal subunit.
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]
UniProt Code:	P46779
NCBI GenInfo Identifier:	13904866
NCBI Gene ID:	6158
NCBI Accession:	NP_000982.2
UniProt Secondary Accession:	P46779,Q6IAY0, Q96FX1, Q9BWQ0, B2R4A6, B4DEP9, C9JB50 E9PB24, G5E9L2,
UniProt Related Accession:	P46779
Molecular Weight:	43kD
NCBI Full Name:	60S ribosomal protein L28 isoform 2
NCBI Synonym Full Names:	ribosomal protein L28
NCBI Official Symbol:	RPL28
NCBI Official Synonym Symbols:	L28
NCBI Protein Information:	60S ribosomal protein L28
UniProt Protein Name:	60S ribosomal protein L28
UniProt Synonym Protein Names:	Large ribosomal subunit protein eL28
Protein Family:	60S ribosomal protein
UniProt Gene Name:	RPL28

	Western blot analysis of extracts of various cell lines, using RPL28 antibody (CAB15095) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of L929 cells using RPL28 antibody (CAB15095) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

RPL28 Antibody (PACO01440)

RPL28 Antibody (PACO11921)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)