Anti-RPS13 Antibody (CAB15720)

Antibody Name:	Anti-RPS13 Antibody
Antibody SKU:	CAB15720
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 79-151 of human RPS13 (NP_001008.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, U-87MG, BxPC-3, LO2, Mouse uterus, Mouse liver, Mouse brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 79-151 of human RPS13 (NP_001008.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GLAP DLPE DLYH LIKK AVAV RKHL ERNR KDKD AKFR LILI ESRI HRLA RYYK TKRV LPPN WKYE SSTA SALV A
Gene ID:	6207
Uniprot:	P62277
Cellular Location:
Calculated MW:	17kDa
Observed MW:	17kDa

Synonyms:

RPS13, S13

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S15P family of ribosomal proteins. It is located in the cytoplasm. The protein has been shown to bind to the 5.8S rRNA in rat. The gene product of the E. coli ortholog (ribosomal protein S15) functions at early steps in ribosome assembly. This gene is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPS13: a ribosomal protein of the S15P family. Located in the cytoplasm and nucleus. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 40S subunit. Binds to the 5.8S rRNA.
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 11p15 Cellular Component: cytosol; focal adhesion; membrane; nucleolus; nucleus; ribosome Molecular Function:mRNA binding; protein binding; structural constituent of ribosome Biological Process: cellular protein metabolic process; gene expression; mRNA catabolic process, nonsense-mediated decay; negative regulation of RNA splicing; selenium metabolic process; selenocysteine metabolic process; SRP-dependent cotranslational protein targeting to membrane; translation; translational elongation; translational initiation; translational termination; viral infectious cycle; viral reproduction; viral transcription
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S15P family of ribosomal proteins. It is located in the cytoplasm. The protein has been shown to bind to the 5.8S rRNA in rat. The gene product of the E. coli ortholog (ribosomal protein S15) functions at early steps in ribosome assembly. This gene is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P62277
NCBI GenInfo Identifier:	50403608
NCBI Gene ID:	6207
NCBI Accession:	P62277.2
UniProt Secondary Accession:	P62277,P19116, Q02546, Q29200, Q498Y0, B2R549,
UniProt Related Accession:	P62277
Molecular Weight:	17,222 Da
NCBI Full Name:	40S ribosomal protein S13
NCBI Synonym Full Names:	ribosomal protein S13
NCBI Official Symbol:	RPS13
NCBI Official Synonym Symbols:	S13
NCBI Protein Information:	40S ribosomal protein S13
UniProt Protein Name:	40S ribosomal protein S13
Protein Family:	40S ribosomal protein
UniProt Gene Name:	RPS13
UniProt Entry Name:	RS13_HUMAN

	Western blot analysis of extracts of various cell lines, using RPS13 antibody (CAB15720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Immunohistochemistry of paraffin-embedded human placenta using RPS13 antibody (CAB15720) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using RPS13 antibody (CAB15720) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using RPS13 antibody (CAB15720) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

RPS13 Antibody (PACO04966)

RPS13 Antibody (PACO11943)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)