The RUFY1 Antibody (CAB7836) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, created by Assay Genie, is highly specific and reactive with human samples, making it ideal for studies in cell biology and cancer research.Rufy1, also known as RUN and FYVE domain containing 1, is known to play a role in the regulation of cell migration and invasion, making it a potential target for therapeutic intervention in cancer metastasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
RUFY1 Antibody
SKU:
CAB7836
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RABIP4, ZFYVE12, RUFY1
Positive Sample:
Mouse kidney, Mouse heart, Rat lung
Cellular Localization:
Cytoplasm, Early Endosome Membrane, Peripheral Membrane Protein.
Calculated MW:
80kDa
Observed MW:
80kDa
This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
80230
RRID
AB_2772129
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RUFY1 Rabbit pAb (CAB7836) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
