Western blot analysis of 293F and S100A12/CGRP 293F trasfected cells, using S100A12/CGRP Rabbit mAb (CAB23912) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The S100A12/CGRP Monoclonal Antibody (CAB23912) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using advanced monoclonal antibody technology, exhibits high specificity and sensitivity for detecting S100A12 and CGRP proteins in samples, making it ideal for use in a variety of experimental applications such as immunohistochemistry, ELISA, and flow cytometry.S100A12 is a member of the S100 family of calcium-binding proteins and has been implicated in inflammation, immune regulation, and various disease processes. CGRP, on the other hand, is a neuropeptide involved in pain transmission, vasodilation, and neurogenic inflammation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
S100A12/CGRP Monoclonal Antibody
SKU:
CAB23912
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC61785
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
MTKLE EHLEG IVNIF HQYSV RKGHF DTLSK GELKQ LLTKE LANTI KNIKD KAVID EIFQG LDANQ DEQVD FQEFI SLVAI ALKAA HYHTH KE
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:1000 - 1:6000
IF/ICC
1:200-1:800
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity.
Purification Method
Affinity purification
Gene ID
6283
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with S100A12/CGRP, using S100A12/CGRP Rabbit mAb (CAB23912) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
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