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Anti-SAMM50 Antibody (CAB3401)

SKU:
CAB3401
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 8 Anti-SAMM50 Antibody CAB3401
  • Cell Biology Antibodies 8 Anti-SAMM50 Antibody CAB3401
  • Cell Biology Antibodies 8 Anti-SAMM50 Antibody CAB3401
  • Cell Biology Antibodies 8 Anti-SAMM50 Antibody CAB3401
  • Cell Biology Antibodies 8 Anti-SAMM50 Antibody CAB3401
€139 - €419
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Description

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Antibody Name:Anti-SAMM50 Antibody
Antibody SKU:CAB3401
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SAMM50 (NP_056195.3).
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, A-431, LO2, HL-60, HT-1080, Mouse eye, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SAMM50 (NP_056195.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGTV HARS LEPL PSSG PDFG GLGE EAEF VEVE PEAK QEIL ENKD VVVQ HVHF DGLG RTKD DIII CEIG DVFK AKNL IEVM RKSH EARE KLLR LGIF RQVD VLID TCQG DDAL PNGL DVTF EVTE LRRL TGSY NTMV GNNE GSMV LGLK LPNL LGRA EKVT FQFS YGTK ETSY GLSF FKPR PGNF ERNF SVNL YKVT GQFP WSSL RETD RGMS AEYS FPIW
Gene ID:25813
Uniprot:Q9Y512
Cellular Location:Cytoplasm, Mitochondrion, Mitochondrion outer membrane, Multi-pass membrane protein
Calculated MW:51kDa
Observed MW:55kDa
Synonyms:SAMM50, CGI-51, OMP85, SAM50, TOB55, TRG-3, YNL026W
Background:This gene encodes a component of the Sorting and Assembly Machinery (SAM) of the mitochondrial outer membrane. The Sam complex functions in the assembly of beta-barrel proteins into the outer mitochondrial membrane.
UniProt Protein Function:SAMM50: May be required for the assembly pathway of mitochondrial outer membrane proteins. Belongs to the SAM50/omp85 family.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Mitochondrial

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: mitochondrial outer membrane; mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function:protein binding

Biological Process: cellular protein metabolic process; protein targeting to mitochondrion; protein import into mitochondrial outer membrane

NCBI Summary:This gene encodes a component of the Sorting and Assembly Machinery (SAM) of the mitochondrial outer membrane. The Sam complex functions in the assembly of beta-barrel proteins into the outer mitochondrial membrane.[provided by RefSeq, Jun 2011]
UniProt Code:Q9Y512
NCBI GenInfo Identifier:118572715
NCBI Gene ID:25813
NCBI Accession:Q9Y512.3
UniProt Related Accession:Q9Y512
Molecular Weight:~ 52kDa
NCBI Full Name:Sorting and assembly machinery component 50 homolog
NCBI Synonym Full Names:SAMM50 sorting and assembly machinery component
NCBI Official Symbol:SAMM50  
NCBI Official Synonym Symbols:OMP85; SAM50; TOB55; TRG-3; CGI-51; YNL026W  
NCBI Protein Information:sorting and assembly machinery component 50 homolog
UniProt Protein Name:Sorting and assembly machinery component 50 homolog
UniProt Synonym Protein Names:Transformation-related gene 3 protein; TRG-3
Protein Family:Sorting and assembly machinery component
UniProt Gene Name:SAMM50  
UniProt Entry Name:SAM50_HUMAN
Anti-SAMM50 Antibody (CAB3401)Western blot analysis of extracts of various cell lines, using SAMM50 antibody (CAB3401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-SAMM50 Antibody (CAB3401)Immunohistochemistry of paraffin-embedded rat heart using SAMM50 Rabbit pAb (CAB3401) at dilution of 1:200 (40x lens).
Anti-SAMM50 Antibody (CAB3401)Immunohistochemistry of paraffin-embedded human liver cancer using SAMM50 Rabbit pAb (CAB3401) at dilution of 1:200 (40x lens).
Anti-SAMM50 Antibody (CAB3401)Immunohistochemistry of paraffin-embedded mouse heart using SAMM50 Rabbit pAb (CAB3401) at dilution of 1:200 (40x lens).
Anti-SAMM50 Antibody (CAB3401)Immunoprecipitation analysis of 600ug extracts of Mouse heart cells using 3ug SAMM50 antibody (CAB3401). Western blot was performed from the immunoprecipitate using SAMM50 antibody (CAB3401) at a dilition of 1:1000.

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