Anti-SEC23IP Antibody (CAB15398)

Antibody Name:	Anti-SEC23IP Antibody
Antibody SKU:	CAB15398
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 600-940 of human SEC23IP (NP_009121.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 600-940 of human SEC23IP (NP_009121.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NLSK CPGP LAVA NGVV KQLH FQEK QMPE EPKL TLDE SYDL VVEN KEVL TLQE TLEA LSLS EYFS TFEK EKID MESL LMCT VDDL KEMG IPLG PRKK IANF VEHK AAKL KKAA SEKK AVAA TSTK GQEQ SAQK TKDM ASLP SESN EPKR KLPV GACV SSVC VNYE SFEV GAGQ VSVA YNSL DFEP EIFF ALGS PIAM FLTI RGVD RIDE NYSL PTCK GFFN IYHP LDPV AYRL EPMI VPDL DLKA VLIP HHKG RKRL HLEL KESL SRMG SDLK QGFI SSLK SAWQ TLNE FARA HTSS TQLQ EELE KVAN QIKE EEEK QVVE AEKV VESP DFSK DEDY LGKV G
Gene ID:	11196
Uniprot:	Q9Y6Y8
Cellular Location:	COPII-coated vesicle membrane, Cytoplasmic side, Cytoplasmic vesicle, Endoplasmic reticulum, Peripheral membrane protein
Calculated MW:	102kDa/111kDa
Observed MW:	Refer to figures

Synonyms:

SEC23IP, MSTP053, P125, P125A

Background:

This gene encodes a member of the phosphatidic acid preferring-phospholipase A1 family. The encoded protein is localized to endoplasmic reticulum exit sites and plays a critical role in ER-Golgi transport as part of the multimeric coat protein II complex. An orthologous gene in frogs is required for normal neural crest cell development, suggesting that this gene may play a role in Waardenburg syndrome neural crest defects. Alternatively spliced transcript variants have been observed for this gene.

UniProt Protein Function:	SEC23IP: Plays a role in the organization of endoplasmic reticulum exit sites. Belongs to the PA-PLA1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Endoplasmic reticulum Chromosomal Location of Human Ortholog: 10q25-q26 Cellular Component: cis-Golgi network; intracellular membrane-bound organelle; cytoplasm; ER-Golgi intermediate compartment Molecular Function:metal ion binding Biological Process: acrosome formation; intracellular protein transport; single fertilization; Golgi organization and biogenesis
NCBI Summary:	This gene encodes a member of the phosphatidic acid preferring-phospholipase A1 family. The encoded protein is localized to endoplasmic reticulum exit sites and plays a critical role in ER-Golgi transport as part of the multimeric coat protein II complex. An orthologous gene in frogs is required for normal neural crest cell development, suggesting that this gene may play a role in Waardenburg syndrome neural crest defects. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Feb 2011]
UniProt Code:	Q9Y6Y8
NCBI GenInfo Identifier:	55584014
NCBI Gene ID:	11196
NCBI Accession:	Q9Y6Y8.1
UniProt Related Accession:	Q9Y6Y8
Molecular Weight:	102kDa; 111kDa
NCBI Full Name:	SEC23-interacting protein
NCBI Synonym Full Names:	SEC23 interacting protein
NCBI Official Symbol:	SEC23IP
NCBI Official Synonym Symbols:	P125; P125A; MSTP053
NCBI Protein Information:	SEC23-interacting protein
UniProt Protein Name:	SEC23-interacting protein
UniProt Synonym Protein Names:	p125
Protein Family:	SEC23-interacting protein
UniProt Gene Name:	SEC23IP
UniProt Entry Name:	S23IP_HUMAN

	Western blot analysis of extracts of various cell lines, using SEC23IP antibody (CAB15398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded Human liver cancer using SEC23IP Rabbit pAb (CAB15398) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using SEC23IP antibody (CAB15398) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using SEC23IP antibody (CAB15398) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using SEC23IP antibody (CAB15398) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

SEC23IP Antibody (PACO12082)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)