Anti-SECISBP2 Antibody (CAB6736)

Antibody Name:	Anti-SECISBP2 Antibody
Antibody SKU:	CAB6736
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 585-854 of human SECISBP2 (NP_076982.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	MCF7, BT-474, HeLa, 293T, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 585-854 of human SECISBP2 (NP_076982.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DELI STPS VEDK SEEP PGTE LQRD TEAS HLAP NHTT FPKI HSRR FRDY CSQM LSKE VDAC VTDL LKEL VRFQ DRMY QKDP VKAK TKRR LVLG LREV LKHL KLKK LKCV IISP NCEK IQSK GGLD DTLH TIID YACE QNIP FVFA LNRK ALGR SLNK AVPV SVVG IFSY DGAQ DQFH KMVE LTVA ARQA YKTM LENV QQEL VGEP RPQA PPSL PTQG PSCP AEDG PPAL KEKE EPHY IEIW KKHL EAYS GCTL ELEE SLEA STSQ MMNL NL
Gene ID:	79048
Uniprot:	Q96T21
Cellular Location:	Mitochondrion, Nucleus
Calculated MW:	87kDa/95kDa
Observed MW:	110kDa

Synonyms:

SECISBP2, SBP2

Background:

The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	SECISBP2: Binds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB. Defects in SECISBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM). This phenotype is associated with a reduction in type II iodothyronine deiodinase activity. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Translation Chromosomal Location of Human Ortholog: 9q22.2 Cellular Component: nucleoplasm; mitochondrion; ribonucleoprotein complex Molecular Function:ribonucleoprotein binding; mRNA 3'-UTR binding Biological Process: selenocysteine incorporation Disease: Thyroid Hormone Metabolism, Abnormal
NCBI Summary:	The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. The gene described in this record encodes a nuclear protein that functions as a SECIS binding protein. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
UniProt Code:	Q96T21
NCBI GenInfo Identifier:	52788293
NCBI Gene ID:	79048
NCBI Accession:	Q96T21.2
UniProt Secondary Accession:	Q96T21,Q5HYY1, Q7L1Z0, Q8IYC0, Q9H0A1, F8W892,
UniProt Related Accession:	Q96T21
Molecular Weight:	854
NCBI Full Name:	Selenocysteine insertion sequence-binding protein 2
NCBI Synonym Full Names:	SECIS binding protein 2
NCBI Official Symbol:	SECISBP2
NCBI Official Synonym Symbols:	SBP2
NCBI Protein Information:	selenocysteine insertion sequence-binding protein 2
UniProt Protein Name:	Selenocysteine insertion sequence-binding protein 2
Protein Family:	Selenocysteine insertion sequence-binding protein
UniProt Gene Name:	SECISBP2
UniProt Entry Name:	SEBP2_HUMAN

	Western blot analysis of extracts of various cell lines, using SECISBP2 antibody (CAB6736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5min.
	Immunohistochemistry of paraffin-embedded human liver damage using SECISBP2 antibody (CAB6736) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using SECISBP2 antibody (CAB6736) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of HeLa cells using SECISBP2 antibody (CAB6736).

Antibodies

SECISBP2 Antibody (PACO12090)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)