Anti-SERPINA3 Antibody (CAB2803)

Antibody Name:	Anti-SERPINA3 Antibody
Antibody SKU:	CAB2803
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-423 of human SERPINA3 (NP_001076.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-423 of human SERPINA3 (NP_001076.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HPNS PLDE ENLT QENQ DRGT HVDL GLAS ANVD FAFS LYKQ LVLK APDK NVIF SPLS ISTA LAFL SLGA HNTT LTEI LKGL KFNL TETS EAEI HQSF QHLL RTLN QSSD ELQL SMGN AMFV KEQL SLLD RFTE DAKR LYGS EAFA TDFQ DSAA AKKL INDY VKNG TRGK ITDL IKDL DSQT MMVL VNYI FFKA KWEM PFDP QDTH QSRF YLSK KKWV MVPM MSLH HLTI PYFR DEEL SCTV VELK YTGN ASAL FILP DQDK MEEV EAML LPET LKRW RDSL EFRE IGEL YLPK FSIS RDYN LNDI LLQL GIEE AFTS KADL SGIT GARN LAVS QVVH KAVL DVFE EGTE ASAA TAVK ITLL SALV ETRT IVRF NRPF LMII VPTD TQNI FFMS KVTN PKQA
Gene ID:	12
Uniprot:	P01011
Cellular Location:	Secreted
Calculated MW:	10kDa/24kDa/47kDa
Observed MW:	48kDa

Synonyms:

SERPINA3, AACT, ACT, GIG24, GIG25

Background:

The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.

UniProt Protein Function:	SERPINA3: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 14q32.1 Cellular Component: extracellular space; extracellular region; intracellular; nucleus Molecular Function:serine-type endopeptidase inhibitor activity; protein binding; DNA binding Biological Process: regulation of lipid metabolic process; maintenance of gastrointestinal epithelium; acute-phase response; inflammatory response
NCBI Summary:	The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. [provided by RefSeq, Jul 2008]
UniProt Code:	P01011
NCBI GenInfo Identifier:	112874
NCBI Gene ID:	12
NCBI Accession:	P01011.2
UniProt Related Accession:	P01011
Molecular Weight:
NCBI Full Name:	Alpha-1-antichymotrypsin
NCBI Synonym Full Names:	serpin family A member 3
NCBI Official Symbol:	SERPINA3
NCBI Official Synonym Symbols:	ACT; AACT; GIG24; GIG25
NCBI Protein Information:	alpha-1-antichymotrypsin
UniProt Protein Name:	Alpha-1-antichymotrypsin
UniProt Synonym Protein Names:	Cell growth-inhibiting gene 24/25 protein; Serpin A3Alpha-1-antichymotrypsin His-Pro-less
Protein Family:	Agmatine coumaroyltransferase
UniProt Gene Name:	SERPINA3
UniProt Entry Name:	AACT_HUMAN

	Western blot analysis of extracts of various cell lines, using SERPINA3 antibody (CAB2803) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat liver using SERPINA3 antibody (CAB2803) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using SERPINA3 antibody (CAB2803) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using SERPINA3 antibody (CAB2803) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using SERPINA3 Rabbit pAb (CAB2803) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-SERPINA3 Antibody (CAB1021)	Human Alpha-1-antichymotrypsin / SERPINA3 ELISA Kit
Anti-alpha 1 Antichymotrypsin Antibody (CAB11364)	Human alpha1ACT (Alpha1 Antichymotrypsin) CLIA Kit (HUES00181)
SERPINA3 Antibody (PACO02077)	Human alpha1ACT (Alpha1 Antichymotrypsin) ELISA Kit (HUES01480)
	Human Alpha-1-antichymotrypsin (SERPINA3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)