The Siglec1 Polyclonal Antibody (CAB22677) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has a high affinity for Siglec1 and is validated for use in various applications such as Western blotting. By specifically binding to Siglec1, researchers can accurately detect and analyze this important protein in a range of cell types.Siglec1, also known as CD169, is a sialic acid binding lectin that plays a crucial role in immune response modulation and host-pathogen interactions. Its expression is often associated with antigen-presenting cells and has been implicated in various disease processes, including viral infections and inflammatory disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
Siglec1 Polyclonal Antibody
SKU:
CAB22677
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Sn, Cd169, Siglec-1, Siglec1
Positive Sample:
Rat spleen
Cellular Localization:
Early Endosome, Extracellular Region, Late Endosome, Plasma Membrane.
Calculated MW:
183kDa
Observed MW:
200kDa
Predicted to enable virion binding activity. Acts upstream of or within positive regulation of T cell apoptotic process and positive regulation of extrinsic apoptotic signaling pathway. Predicted to be located in extracellular region and membrane. Predicted to be integral component of membrane. Predicted to be active in early endosome; late endosome; and plasma membrane. Is expressed in immune system; reproductive system; and skin. Orthologous to human SIGLEC1 (sialic acid binding Ig like lectin 1).
Purification Method
Affinity purification
Gene ID
20612
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat spleen using Siglec1 Rabbit pAb (CAB22677) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
