The SIGLEC15 Antibody (CAB18660) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody is highly specific and reacts with human samples, making it ideal for Western blot applications to detect and analyze Siglec15 expression in various cell types.Siglec15 is a member of the Siglec family of proteins, which are known to play a critical role in immune responses and inflammation regulation. Research has shown that Siglec15 is upregulated in certain types of cancer, suggesting a role in tumor immune evasion.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
SIGLEC15 Antibody
SKU:
CAB18660
Size:
20μL, 100μL
Reactivity:
Human, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD33L3, HsT1361, SIGLEC-15, Siglec-15
Positive Sample:
HUVEC, Rat brain
Cellular Localization:
Plasma Membrane.
Calculated MW:
36kDa
Observed MW:
36kDa
Predicted to be involved in regulation of actin cytoskeleton organization; regulation of bone resorption; and regulation of osteoclast development. Predicted to be integral component of membrane. Predicted to be part of protein-containing complex. Predicted to be active in plasma membrane.
Purification Method
Affinity purification
Gene ID
284266
RRID
AB_2862397
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Siglec-15 Rabbit pAb (CAB18660) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human spleen using Siglec-15 Rabbit pAb (CAB18660) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded human spleen using Siglec-15 Rabbit pAb (CAB18660) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
