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Anti-SIK2 Antibody (CAB8321)

SKU:
CAB8321
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

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Antibody Name:SIK2 Rabbit Polyclonal Antibody
Antibody SKU:CAB8321
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 827-926 of human SIK2 (NP_056006.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, SW620, A-549, HeLa, Jurkat, HepG2, Mouse fat, Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 827-926 of human SIK2 (NP_056006.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PPPP PPRQ PGAA PAPL QFSY QTCE LPSA ASPA PDYP TPCQ YPVD GAQQ SDLT GPDC PRSP GLQE APSS YDPL ALSE LPGL FDCE MLDA VDPQ HNGY VLVN
Gene ID:23235
Uniprot:Q9H0K1
Cellular Location:Cytoplasm
Calculated MW:103kDa
Observed MW:129kDa
Synonyms:SIK2, LOH11CR1I, QIK, SIK-2, SNF1LK2
Background:
UniProt Protein Function:QIK: a serine/threonine kinase of the CAMKL family and related to AMPK. Specifically expressed in adipose tissues. Like AMPK, QIK is phosphorylated and activated by LKB1. Part of a molecular complex including TORC2 and calcineurin that regulates the effects of circulating glucose and gut hormones during feeding on TORC2-mediated gene expression. In mice, insulin promotes the phosphorylation and ubiquitin-dependent degradation of TORC2, inhibiting gluconeogenic gene expression during re-feeding. In response to insulin, Akt2 phosphorylates and activates QIK which in turn stimulates the phosphorylation and cytoplasmic translocation of TORC2 where it associates with COP1, which promotes its ubiquitination and proteasomal degradation. Phosphorylates Ser794 of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3.
UniProt Protein Details:

Protein type:Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; CAMK group; CAMKL family; QIK subfamily

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytoplasm; nucleus

Molecular Function:ATP binding; magnesium ion binding; protein binding; protein serine/threonine kinase activity

Biological Process: insulin receptor signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of insulin receptor signaling pathway

UniProt Code:Q9H0K1
NCBI GenInfo Identifier:59798973
NCBI Gene ID:23235
NCBI Accession:Q9H0K1.1
UniProt Secondary Accession:Q9H0K1,O94878, Q17RV0, Q6AZE2, Q76N03, Q8NCV7, Q96CZ8 A8K5B8, B0YJ94,
UniProt Related Accession:Q9H0K1
Molecular Weight:103,915 Da
NCBI Full Name:Serine/threonine-protein kinase SIK2
NCBI Synonym Full Names:salt inducible kinase 2
NCBI Official Symbol:SIK2  
NCBI Official Synonym Symbols:QIK; SIK-2; SNF1LK2; LOH11CR1I  
NCBI Protein Information:serine/threonine-protein kinase SIK2
UniProt Protein Name:Serine/threonine-protein kinase SIK2
UniProt Synonym Protein Names:Qin-induced kinase; Salt-inducible kinase 2; SIK-2; Serine/threonine-protein kinase SNF1-like kinase 2
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:SIK2  
UniProt Entry Name:SIK2_HUMAN
Anti-SIK2 Polyclonal Antibody (CAB8321)Western blot analysis of extracts of various cell lines, using SIK2 antibody (CAB8321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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