Anti-SKP1 Antibody (CAB2566)

Antibody Name:	Anti-SKP1 Antibody
Antibody SKU:	CAB2566
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human SKP1 (NP_733779.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	A-549, A-431, HeLa, LO2, SW480, K-562, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human SKP1 (NP_733779.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPSI KLQS SDGE IFEV DVEI AKQS VTIK TMLE DLGM DDEG DDDP VPLP NVNA AILK KVIQ WCTH HKDD PPPP EDDE NKEK RTDD IPVW DQEF LKVD QGTL FELI LAAN YLDI KGLL DVTC KTVA NMIK GKTP EEIR KTFN IKND FTEE EEAQ VRKE NQWC EEK
Gene ID:	6500
Uniprot:	P63208
Cellular Location:
Calculated MW:	18kDa
Observed MW:	19kDa

Synonyms:

SKP1, EMC19, OCP-II, OCP2, SKP1A, TCEB1L, p19A

Background:

This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

UniProt Protein Function:	SKP1A: Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110. Component of an E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Component of the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC. Part of a SCF(BTRC)-like complex lacking CUL1, which is associated with phosphorylated NFKBIA and RELA; RELA interacts directly with NFKBIA. Component of the SCF(FBXO44) complex, composed of SKP1, CUL1 and FBXO44. Identified in a complex with SKP2, CKS1B and p27Kip1. Interacts with the cyclin A/CDK2 complex. Part of a SCF- like complex consisting of CUL7, RBX1, SKP1 and FBXW8. Part of several SCF complexes containing SKP1 and one of the F-box proteins. Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts with FBXO2, FBXO4, FBXW7 and TRIM21. Interacts with FBXO45. Component of the SCF(FBXO17) complex, composed of SKP1, CUL1 and FBXO17. Component of the SCF(FBXO27) complex, composed of SKP1, CUL1 and FBXO27. Component of the SCF(Cyclin F) complex consisting of CUL1, RBX1, SKP1 and CCNF. Belongs to the SKP1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; Cell cycle regulation Chromosomal Location of Human Ortholog: 5q31 Cellular Component: nucleoplasm; SCF ubiquitin ligase complex; PcG protein complex; cytosol Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; circadian rhythm; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; viral reproduction; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; mitotic cell cycle; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle
NCBI Summary:	This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq, Jul 2008]
UniProt Code:	P63208
NCBI GenInfo Identifier:	52783797
NCBI Gene ID:	6500
NCBI Accession:	P63208.2
UniProt Secondary Accession:	P63208,P34991, Q8TAY2, D3DQ97, D3DQ98,
UniProt Related Accession:	P63208
Molecular Weight:	163
NCBI Full Name:	S-phase kinase-associated protein 1
NCBI Synonym Full Names:	S-phase kinase-associated protein 1
NCBI Official Symbol:	SKP1
NCBI Official Synonym Symbols:	OCP2; p19A; EMC19; SKP1A; OCP-II; TCEB1L
NCBI Protein Information:	S-phase kinase-associated protein 1; SIII; OCP-2; p19skp1; organ of Corti protein 2; organ of Corti protein II; cyclin A/CDK2-associated p19; cyclin A/CDK2-associated protein p19; cyclin-A/CDK2-associated protein p19; transcription elongation factor B polypeptide 1-like; RNA polymerase II elongation factor-like protein OCP2
UniProt Protein Name:	S-phase kinase-associated protein 1
UniProt Synonym Protein Names:	Cyclin-A/CDK2-associated protein p19; p19A; Organ of Corti protein 2; OCP-2; Organ of Corti protein II; OCP-II; RNA polymerase II elongation factor-like protein; SIII; Transcription elongation factor B polypeptide 1-like; p19skp1
Protein Family:	SKP1-like protein
UniProt Gene Name:	SKP1
UniProt Entry Name:	SKP1_HUMAN

	Western blot analysis of extracts of various cell lines, using SKP1 antibody (CAB2566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded mouse lung using SKP1 antibody (CAB2566) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-SKP1 Antibody (CAB12505)	SKP1A/p19 Colorimetric Cell-Based ELISA Kit
Anti-SKP1 Antibody (CAB12518)
Anti-Skp1 Antibody (CAB9021)
SKP1 Antibody (PACO01503)
SKP1 Antibody (PACO12232)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)