The SKP1 Monoclonal Antibody (CAB9021) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is recommended for use in Western blot and immunohistochemistry applications, allowing for the detection and analysis of SKP1 protein in various experimental settings.SKP1 is essential for the proper function of the SCF complex, which plays a crucial role in ubiquitin-mediated protein degradation. Aberrant regulation of this complex has been implicated in various diseases, including cancer and neurodegenerative disorders.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SKP1 Monoclonal Antibody
SKU:
CAB9021
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1384
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
Purification Method
Affinity purification
Gene ID
6500
RRID
AB_2863642
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SKP1 Rabbit mAb (CAB9021) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Confocal imaging of NIH/3T3 cells using SKP1 Rabbit mAb (CAB9021, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (CABS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
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