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Anti-SLC12A2 Antibody (CAB11675)

SKU:
CAB11675
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
  • Signal Transduction Antibodies 1 Anti-SLC12A2 Antibody CAB11675
  • Signal Transduction Antibodies 1 Anti-SLC12A2 Antibody CAB11675
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-SLC12A2 Antibody
Antibody SKU:CAB11675
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human SLC12A2 (NP_001037.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse pancreas, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human SLC12A2 (NP_001037.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GEAS GESE PAKG SEEA KGRF RVNF VDPA ASSS AEDS LSDA AGVG VDGP NVSF QNGG DTVL SEGS SLHS GGGG GSGH HQHY YYDT HTNT YYLR TFGH NTMD AVPR IDHY RHTA AQLG EKLL RPSL AELH DELE KEPF EDGF ANGE ESTP TRDA VVTY TAES K
Gene ID:6558
Uniprot:P55011
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:129kDa/131kDa
Observed MW:170kDa
Synonyms:SLC12A2, BSC, BSC2, NKCC1, PPP1R141
Background:The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene.
UniProt Protein Function:NKCC1: a multi-pass membrane cation transporter protein. Aids transcellular movement of chloride across both secretory and absorptive epithelia. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Expressed in many tissues. Bumetanide is a potent NKCC1 antagonist. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 5q23.3

Cellular Component: membrane; basolateral plasma membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; vesicle

Molecular Function:protein binding; ammonium transmembrane transporter activity; sodium:potassium:chloride symporter activity

Biological Process: hyperosmotic response; transport; multicellular organism growth; transepithelial chloride transport; sodium ion transport; ion transport; detection of mechanical stimulus involved in sensory perception of sound; positive regulation of cell volume; transmembrane transport; gamma-aminobutyric acid signaling pathway; potassium ion transport; ammonium transport

NCBI Summary:The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:P55011
NCBI GenInfo Identifier:1709292
NCBI Gene ID:6558
NCBI Accession:P55011.1
UniProt Secondary Accession:P55011,P55012,
UniProt Related Accession:P55011
Molecular Weight:1212
NCBI Full Name:Solute carrier family 12 member 2
NCBI Synonym Full Names:solute carrier family 12 (sodium/potassium/chloride transporter), member 2
NCBI Official Symbol:SLC12A2  
NCBI Official Synonym Symbols:BSC; BSC2; NKCC1; PPP1R141  
NCBI Protein Information:solute carrier family 12 member 2; basolateral Na-K-Cl symporter; protein phosphatase 1, regulatory subunit 141; bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; solute carrier family 12 (sodium/potassium/chloride transporters), member 2
UniProt Protein Name:Solute carrier family 12 member 2
UniProt Synonym Protein Names:Basolateral Na-K-Cl symporter; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
UniProt Gene Name:SLC12A2  
UniProt Entry Name:S12A2_HUMAN
Anti-SLC12A2 Antibody (CAB11675)Western blot analysis of extracts of mouse pancreas, using SLC12A2 antibody (CAB11675) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-SLC12A2 Antibody (CAB11675)Western blot analysis of extracts of rat brain, using SLC12A2 antibody (CAB11675) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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