Anti-SLC1A5 Antibody (CAB6981)

Antibody Name:	Anti-SLC1A5 Antibody
Antibody SKU:	CAB6981
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 154-224 of human SLC1A5 (NP_005619.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:3000
Reactivity:	Human
Positive Samples:	NCI-H460, U-251MG, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 154-224 of human SLC1A5 (NP_005619.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QPGA ASAA INAS VGAA GSAE NAPS KEVL DSFL DLAR NIFP SNLV SAAF RSYS TTYE ERNI TGTR VKVP VGQ
Gene ID:	6510
Uniprot:	Q15758
Cellular Location:	Cell membrane, Melanosome, Multi-pass membrane protein
Calculated MW:	33kDa/36kDa/56kDa
Observed MW:	50kDa

Synonyms:	SLC1A5, AAAT, ASCT2, ATBO, M7V1, M7VS1, R16, RDRC
Background:

UniProt Protein Function:	SLC1A5: Has a broad substrate specificity, a preference for zwitterionic amino acids, and a sodium-dependence. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated amino acids, anionic amino acids, and cationic amino acids. Act as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses. Placenta, lung, skeletal muscle, kidney, pancreas, and intestine. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A5 subfamily. 1 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:	Protein type:Transporter; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: Golgi apparatus; integral to plasma membrane; membrane; plasma membrane Molecular Function:amino acid transmembrane transporter activity; L-glutamine transmembrane transporter activity; neutral amino acid transmembrane transporter activity; protein binding; receptor activity Biological Process: amino acid transport; glutamine transport; neutral amino acid transport
NCBI Summary:	The SLC1A5 gene encodes a sodium-dependent neutral amino acid transporter that can act as a receptor for RD114/type D retrovirus (Larriba et al., 2001 [PubMed 11781704]).[supplied by OMIM, Jan 2011]
UniProt Code:	Q15758
NCBI GenInfo Identifier:	21542389
NCBI Gene ID:	6510
NCBI Accession:	Q15758.2
UniProt Secondary Accession:	Q15758,O95720, Q96RL9, Q9BWQ3, Q9UNP2, A8K9H5, B4DR77 B4DWS4, B7ZB81, D0EYG6, E9PC01,
UniProt Related Accession:	Q15758
Molecular Weight:	33,710 Da
NCBI Full Name:	Neutral amino acid transporter B(0)
NCBI Synonym Full Names:	solute carrier family 1 member 5
NCBI Official Symbol:	SLC1A5
NCBI Official Synonym Symbols:	R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
NCBI Protein Information:	neutral amino acid transporter B(0)
UniProt Protein Name:	Neutral amino acid transporter B(0)
UniProt Synonym Protein Names:	Baboon M7 virus receptor; RD114/simian type D retrovirus receptor; Sodium-dependent neutral amino acid transporter type 2; Solute carrier family 1 member 5
Protein Family:	Neutral amino acid transporter
UniProt Gene Name:	SLC1A5
UniProt Entry Name:	AAAT_HUMAN

Western blot analysis of extracts of various cell lines, using SLC1A5 antibody (CAB6981) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Antibodies

Anti-SLC1A5 Antibody (CAB12676)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)