Anti-SLC20A1 Antibody (CAB4117)

Antibody Name:	Anti-SLC20A1 Antibody
Antibody SKU:	CAB4117
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 257-356 of human SLC20A1 (NP_005406.3).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, A-431, HeLa, HT-1080, NCI-H460, Mouse liver, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 257-356 of human SLC20A1 (NP_005406.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KIER EIKC SPSE SPLM EKKN SLKE DHEE TKLS VGDI ENKH PVSE VGPA TVPL QAVV EERT VSFK LGDL EEAP ERER LPSV DLKE ETSI DSTV NGAV QLPN
Gene ID:	6574
Uniprot:	Q8WUM9
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	73kDa
Observed MW:	70-100kDa

Synonyms:

SLC20A1, GLVR1, Glvr-1, PIT1, PiT-1

Background:

The protein encoded by this gene is a sodium-phosphate symporter that absorbs phosphate from interstitial fluid for use in cellular functions such as metabolism, signal transduction, and nucleic acid and lipid synthesis. The encoded protein is also a retroviral receptor, causing human cells to be susceptible to infection by gibbon ape leukemia virus, simian sarcoma-associated virus, feline leukemia virus subgroup B, and 10A1 murine leukemia virus.

UniProt Protein Function:	SLC20A1: Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. May play a role in extracellular matrix and cartilage calcification as well as in vascular calcification. May function as a retroviral receptor as it confers human cells susceptibility to infection to Gibbon Ape Leukemia Virus (GaLV), Simian sarcoma-associated virus (SSAV) and Feline leukemia virus subgroup B (FeLV-B) as well as 10A1 murine leukemia virus (10A1 MLV). Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass; Transporter Chromosomal Location of Human Ortholog: 2q13 Cellular Component: membrane; integral to plasma membrane; plasma membrane Molecular Function:signal transducer activity; high affinity inorganic phosphate:sodium symporter activity; receptor activity; sodium:phosphate symporter activity; inorganic phosphate transmembrane transporter activity Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; transport; ion transport; signal transduction; transmembrane transport; phosphate metabolic process
NCBI Summary:	The protein encoded by this gene is a sodium-phosphate symporter that absorbs phosphate from interstitial fluid for use in cellular functions such as metabolism, signal transduction, and nucleic acid and lipid synthesis. The encoded protein is also a retroviral receptor, causing human cells to be susceptible to infection by gibbon ape leukemia virus, simian sarcoma-associated virus, feline leukemia virus subgroup B, and 10A1 murine leukemia virus.[provided by RefSeq, Mar 2011]
UniProt Code:	Q8WUM9
NCBI GenInfo Identifier:	74730735
NCBI Gene ID:	6574
NCBI Accession:	Q8WUM9.1
UniProt Secondary Accession:	Q8WUM9,Q08344, Q6DHX8, Q9UQ82,
UniProt Related Accession:	Q8WUM9
Molecular Weight:	679
NCBI Full Name:	Sodium-dependent phosphate transporter 1
NCBI Synonym Full Names:	solute carrier family 20 (phosphate transporter), member 1
NCBI Official Symbol:	SLC20A1
NCBI Official Synonym Symbols:	PIT1; GLVR1; PiT-1; Glvr-1
NCBI Protein Information:	sodium-dependent phosphate transporter 1; leukemia virus receptor 1 homolog; solute carrier family 20 member 1; gibbon ape leukemia virus receptor 1
UniProt Protein Name:	Sodium-dependent phosphate transporter 1
UniProt Synonym Protein Names:	Gibbon ape leukemia virus receptor 1; GLVR-1; Leukemia virus receptor 1 homolog; Phosphate transporter 1; PiT-1; Solute carrier family 20 member 1
UniProt Gene Name:	SLC20A1
UniProt Entry Name:	S20A1_HUMAN

	Western blot analysis of extracts of various cell lines, using SLC20A1 antibody (CAB4117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of C6 cells using SLC20A1 antibody (CAB4117) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using SLC20A1 antibody (CAB4117) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

SLC20A1 Antibody (PACO12250)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)