The SMARCD2 Antibody (CAB12104) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. It binds to the SMARCD2 protein, allowing for the detection and analysis of this important molecule in various cell types.SMARCD2, also known as BAF60B, is a subunit of the SWI/SNF chromatin remodeling complex, which plays a critical role in controlling gene expression and cell fate determination. Dysregulation of SMARCD2 has been implicated in various diseases, including cancer and developmental disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SMARCD2 Antibody
SKU:
CAB12104
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SGD2, Rsc6p, BAF60B, CRACD2, PRO2451, SMARCD2
Positive Sample:
Mouse thymus, Rat thymus
Cellular Localization:
Nucleus.
Calculated MW:
59kDa
Observed MW:
60kDa
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6603
RRID
AB_2758998
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat thymus, using SMARCD2 Rabbit pAb (CAB12104) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from Mouse thymus, using SMARCD2 Rabbit pAb (CAB12104) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
