Anti-SOD3 Antibody (CAB6984)

Antibody Name:	Anti-SOD3 Antibody
Antibody SKU:	CAB6984
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-160 of human SOD3 (NP_003093.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	22Rv1, HL-60, Mouse kidney, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-160 of human SOD3 (NP_003093.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	WTGE DSAE PNSD SAEW IRDM YAKV TEIW QEVM QRRD DDGA LHAA CQVQ PSAT LDAA QPRV TGVV LFRQ LAPR AKLD AFFA LEGF PTEP NSSS RAIH VHQF GDLS QGCE STGP HYNP LAVP HPQH PGDF GNFA VRDG SLWR YR
Gene ID:	6649
Uniprot:	P08294
Cellular Location:	Secreted, extracellular space
Calculated MW:	25kDa
Observed MW:	35kDa

Synonyms:

SOD3, EC-SOD

Background:

This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the conversion of superoxide radicals into hydrogen peroxide and oxygen, which may protect the brain, lungs, and other tissues from oxidative stress. Proteolytic processing of the encoded protein results in the formation of two distinct homotetramers that differ in their ability to interact with the extracellular matrix (ECM). Homotetramers consisting of the intact protein, or type C subunit, exhibit high affinity for heparin and are anchored to the ECM. Homotetramers consisting of a proteolytically cleaved form of the protein, or type A subunit, exhibit low affinity for heparin and do not interact with the ECM. A mutation in this gene may be associated with increased heart disease risk.

UniProt Protein Function:	SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family.
UniProt Protein Details:	Protein type:EC 1.15.1.1; Secreted, signal peptide; Oxidoreductase; Secreted Chromosomal Location of Human Ortholog: 4p15.2 Cellular Component: extracellular matrix; extracellular space; Golgi lumen; cytoplasm; extracellular region; trans-Golgi network; nucleus Molecular Function:heparin binding; protein binding; copper ion binding; zinc ion binding; superoxide dismutase activity Biological Process: response to reactive oxygen species; response to copper ion; removal of superoxide radicals; response to hypoxia
NCBI Summary:	This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the conversion of superoxide radicals into hydrogen peroxide and oxygen, which may protect the brain, lungs, and other tissues from oxidative stress. Proteolytic processing of the encoded protein results in the formation of two distinct homotetramers that differ in their ability to interact with the extracellular matrix (ECM). Homotetramers consisting of the intact protein, or type C subunit, exhibit high affinity for heparin and are anchored to the ECM. Homotetramers consisting of a proteolytically cleaved form of the protein, or type A subunit, exhibit low affinity for heparin and do not interact with the ECM. A mutation in this gene may be associated with increased heart disease risk. [provided by RefSeq, Oct 2015]
UniProt Code:	P08294
NCBI GenInfo Identifier:	108885292
NCBI Gene ID:	6649
NCBI Accession:	P08294.2
UniProt Related Accession:	P08294
Molecular Weight:
NCBI Full Name:	Extracellular superoxide dismutase
NCBI Synonym Full Names:	superoxide dismutase 3
NCBI Official Symbol:	SOD3
NCBI Official Synonym Symbols:	EC-SOD
NCBI Protein Information:	extracellular superoxide dismutase [Cu-Zn]
UniProt Protein Name:	Extracellular superoxide dismutase [Cu-Zn]
Protein Family:	Sodium channel
UniProt Gene Name:	SOD3
UniProt Entry Name:	SODE_HUMAN

	Western blot analysis of extracts of various cell lines, using SOD3 antibody (CAB6984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human mammary cancer using SOD3 antibody (CAB6984) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat ovary using SOD3 antibody (CAB6984) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse pancreas using SOD3 antibody (CAB6984) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-SOD3 Antibody (CAB13935)	Human EC-SOD / SOD3 ELISA Kit
SOD3 Antibody (PACO12393)	Human Extracellular superoxide dismutase [Cu-Zn] (SOD3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)