Anti-SORD Antibody (CAB2118)

Antibody Name:	Anti-SORD Antibody
Antibody SKU:	CAB2118
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-357 of human SORD (NP_003095.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HepG2, U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-357 of human SORD (NP_003095.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA AKPN NLSL VVHG PGDL RLEN YPIP EPGP NEVL LRMH SVGI CGSD VHYW EYGR IGNF IVKK PMVL GHEA SGTV EKVG SSVK HLKP GDRV AIEP GAPR ENDE FCKM GRYN LSPS IFFC ATPP DDGN LCRF YKHN AAFC YKLP DNVT FEEG ALIE PLSV GIHA CRRG GVTL GHKV LVCG AGPI GMVT LLVA KAMG AAQV VVTD LSAT RLSK AKEI GADL VLQI SKES PQEI ARKV EGQL GCKP EVTI ECTG AEAS IQAG IYAT RSGG NLVL VGLG SEMT TVPL LHAA IREV DIKG VFRY CNTW PVAI SMLA SKSV NVKP LVTH RFPL EKAL EAFE TFKK GLGL KIML KCDP SDQN P
Gene ID:	6652
Uniprot:	Q00796
Cellular Location:	Cell projection, Mitochondrion membrane, Peripheral membrane protein, cilium, flagellum
Calculated MW:	10kDa/38kDa
Observed MW:	38KDa

Synonyms:

SORD, HEL-S-95n, SORD1

Background:

Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.

UniProt Protein Function:	SORD: Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Belongs to the zinc-containing alcohol dehydrogenase family.
UniProt Protein Details:	Protein type:EC 1.1.1.14; Oxidoreductase; Carbohydrate Metabolism - fructose and mannose Chromosomal Location of Human Ortholog: 15q15.3 Cellular Component: extracellular space; membrane; mitochondrial membrane Molecular Function:zinc ion binding; L-iditol 2-dehydrogenase activity; carbohydrate binding; NAD binding Biological Process: sperm motility; L-xylitol metabolic process; L-xylitol catabolic process; glucose metabolic process; fructose biosynthetic process; sorbitol catabolic process
NCBI Summary:	Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
UniProt Code:	Q00796
NCBI GenInfo Identifier:	292495088
NCBI Gene ID:	6652
NCBI Accession:	Q00796.4
UniProt Related Accession:	Q00796
Molecular Weight:
NCBI Full Name:	Sorbitol dehydrogenase
NCBI Synonym Full Names:	sorbitol dehydrogenase
NCBI Official Symbol:	SORD
NCBI Official Synonym Symbols:	SORD1; HEL-S-95n
NCBI Protein Information:	sorbitol dehydrogenase
UniProt Protein Name:	Sorbitol dehydrogenase
UniProt Synonym Protein Names:	L-iditol 2-dehydrogenase
Protein Family:	Serine 3-dehydrogenase
UniProt Gene Name:	SORD
UniProt Entry Name:	DHSO_HUMAN

	Western blot analysis of extracts of various cell lines, using SORD Rabbit pAb (CAB2118) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse kidney using SORD Rabbit pAb (CAB2118) at dilution of 1:25 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using SORD antibody (CAB2118). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-SORD Antibody (CAB15727)	Human SORD / Sorbitol Dehydrogenase ELISA Kit
SORD Antibody (PACO12395)	Human SDH (Sorbitol Dehydrogenase) CLIA Kit (HUES01038)
	Human Sorbitol dehydrogenase (SORD) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)